Potential Rhizospheric Bacteria of Local Rice Rice “Cempo Laut” in Merauke
Abstract
Non-cultivated local rice is known to have high resistance to various environmental stresses. This resistance is inseparable from the role of symbiotic and non-symbiotic rhizosphere bacteria that live in the root rhizosphere. This study aims to isolate and study the potential of rhizobacteria that live around the roots of non-cultivated local rice plants. This research is exploratory research to obtain rhizobacterial isolates that can stimulate plant growth and development and can be applied to cultivated rice plants. Soil samples were taken from Mimi Baru Village, Jagebob District. Sampling was carried out aseptically. Isolation was carried out using the multilevel dilution method. Isolates grown in NA media were then subcultured to obtain pure isolates. The isolates obtained were then characterized morphologically according to Bergey's Manual of Determinative Bacteriology and characterized microscopically. Based on the results of this study it can be concluded that there were 12 bacterial isolates in the local rice "Cempo Laut" which were suspected to be Bacillus and Pseudomonas. The results of this study need to be tested further to determine the effect on plant growth and development.
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Ahemad, M., & Kibret, M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria: Current perspectives. Journal of King Saud University - Science, 26(1), 1–20. https://doi.org/10.1016/j.jksus.2013.05.001
Bai, YC, Chang, YY, Hussain, M., Lu, B., Zhang, JP, Song, XB, Lei, XS, & Pei, D. (2020). Soil chemical and microbiological properties are changed by long-term chemical fertilizers that limit ecosystem functioning. Microorganisms, 8(5). https://doi.org/10.3390/microorganisms8050694
Belkar, Y. ., & Gade, R. . (2012). Biochemical Characterization and Growth Promotion Activities of Pseudomonas Fluorescens. J.Pl.Dis.Sci, 7(2), 170–174.
Bhat, MA, Kumar, V., Bhat, MA, Wani, IA, Dar, FL, Farooq, I., Bhatti, F., Koser, R., Rahman, S., & Jan, AT (2020). Mechanistic Insights of the Interaction of Plant Growth-Promoting Rhizobacteria (PGPR) With Plant Roots Toward Enhancing Plant Productivity by Alleviating Salinity Stress. Frontiers in Microbiology, 11(August), 1–20. https://doi.org/10.3389/fmicb.2020.01952
Bumunang, EW, & Babalola, OO (2014). Characterization of Rhizobacteria from field grown genetically modified (GM) and non-GM maize. Brazilian Archives of Biology and Technology, 57(1), 1–8. https://doi.org/10.1590/S1516-89132014000100001
Chandran, H., & Meena, M. (2021). Plant Growth-Promoting Rhizobacteria as a Green Alternative for Sustainable Agriculture. Sustainability, 13(10986), 1–30. https://doi.org/doi.org/10.3390/su131910986
Chauhan, A., Guleria, S., Balgir, PP, Walia, A., Mahajan, R., Mehta, P., & Shirkot, CK (2016). Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from the rhizosphere of Valeriana jatamansi and its growth promotion. Brazilian Journal of Microbiology, 48(2), 294–304. https://doi.org/10.1016/j.bjm.2016.12.001
Choudhary, DK, Sharma, KP, & Gaur, RK (2011). Biotechnological perspectives of microbes in agro-ecosystems. Biotechnology Letters, 33(10), 1905–1910. https://doi.org/10.1007/s10529-011-0662-0
de Souza, EM, Lamb, TI, Lamb, TA, dos Santos Silva, A., da Fré de Carvalho, S., Nyland, V., Lopes, MCB, Grohs, M., Marconatto, L., dos Anjos Borges , LG, Giongo, A., Granada, CE, & Sperotto, RA (2021). Rhizospheric Soil from Rice Paddy Presents Isolable Bacteria Able to Induce Cold Tolerance in Rice Plants. Journal of Soil Science and Plant Nutrition, 21(3), 1993–2006. https://doi.org/10.1007/s42729-021-00496-y
Diagne, N., Ndour, M., Djighaly, PI, Ngom, D., Ngom, MCN, Ndong, G., Svistoonoff, S., & Cherif-Silini, H. (2020). Effect of Plant Growth Promoting Rhizobacteria (PGPR) and Arbuscular Mycorrhizal Fungi (AMF) on Salt Stress Tolerance of Casuarina obesa (Miq.). Frontiers in Sustainable Food Systems, 4(December), 1–8. https://doi.org/10.3389/fsufs.2020.601004
Ding, LJ, Cui, HL, Nie, SA, Long, XE, Duan, GL, & Zhu, YG (2019). Microbiomes inhabiting rice roots and rhizosphere. FEMS Microbiology Ecology, 95(5), 1–13. https://doi.org/10.1093/femsec/fiz040
Edwards, J., Johnson, C., Santos-Medellín, C., Lurie, E., Podishetty, NK, Bhatnagar, S., Eisen, JA, Sundaresan, V., & Jeffery, LD (2015). Structure, variation, and assembly of the root-associated microbiomes of rice. Proceedings of the National Academy of Sciences of the United States of America, 112(8), E911–E920. https://doi.org/10.1073/pnas.1414592112
Ekowati, NY, Widijastuti, R., & Alang, R. (2018). Root penetration test using wax coating on local and non-local rice in Merauke district (. Agrosains, 5(2), 82–90.
Engrob, KL, Larsen, T., Peixoto, L., & Rasmussen, J. (2020). Gram-positive bacteria control the rapid anabolism of protein-sized soil organic nitrogen compounds questioning the present paradigm. Scientific Reports, 10(1), 1–9. https://doi.org/10.1038/s41598-020-72696-y
Finkel, OM, Castrillo, G., Herrera Paredes, S., Salas González, I., & Dangl, JL (2017). Understanding and exploiting plant beneficial microbes. Current Opinion in Plant Biology, 38, 155–163. https://doi.org/10.1016/j.pbi.2017.04.018
García de Salamone, IE, Funes, JM, Di Salvo, LP, Escobar-Ortega, JS, D'Auria, F., Ferrando, L., & Fernandez-Scavino, A. (2012). Inoculation of paddy rice with Azospirillum brasilense and Pseudomonas fluorescens: Impact of plant genotypes on rhizosphere microbial communities and field crop production. Applied Soil Ecology, 61(2012), 196–204. https://doi.org/10.1016/j.apsoil.2011.12.012
Garcia, J., Gannett, M., Wei, LP, Cheng, L., Hu, S., Sparks, J., Giovannoni, J., & Kao-Kniffin, J. (2022). Selection pressure on the rhizosphere microbiome can alter nitrogen use efficiency and yield seed in Brassica rapa. Communications Biology, 5(1), 1–11. https://doi.org/10.1038/s42003-022-03860-5
Grover, M., Ali, SZ, Sandhya, V., Apostle, A., & Venkateswarlu, B. (2011). Role of microorganisms in adaptation of agricultural crops to abiotic stresses. World Journal of Microbiology and Biotechnology, 27(5), 1231–1240. https://doi.org/10.1007/s11274-010-0572-7
Grover, M., Bodhankar, S., Sharma, A., Sharma, P., Singh, J., & Nain, L. (2021). PGPR Mediated Alterations in Root Traits: Way Toward Sustainable Crop Production. Frontiers in Sustainable Food Systems, 4(January), 1–28. https://doi.org/10.3389/fsufs.2020.618230
Hasan, MK, McInroy, JA, & Kloepper, JW (2019). The International of Rhizodeposits with Plant Growth-Promoting Rhizobacteria in the Rhizosphere : A Review. Agriculture, 9(142), 1–13. https://doi.org/doi:10.3390/agriculture9070142
He, X., Xu, M., Wei, Q., Tang, M., Guan, L., Lou, L., Xu, X., Hu, Z., Chen, Y., Shen, Z., & Xia, Y. (2020). Promotion of growth and phytoextraction of cadmium and lead in Solanum nigrum L. mediated by plant-growth-promoting rhizobacteria. Ecotoxicology and Environmental Safety, 205(September), 111333. https://doi.org/10.1016/j.ecoenv.2020.111333
Hossain, MA, Bhattacharjee, S., Armin, SM, Qian, P., Xin, W., Li, HY, Burritt, DJ, Fujita, M., & Tran, LSP (2015). Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: Insights from ROS detoxification and scavenging. Frontiers in Plant Science, 6(June), 1–19. https://doi.org/10.3389/fpls.2015.00420
Hyder, S., Gondal, AS, Rizvi, ZF, Ahmad, R., Alam, MM, Hannan, A., Ahmed, W., Fatima, N., & Inam-ul-Haq, M. (2020). Characterization of native plant growth promoting rhizobacteria and their anti-oomycete potential against Phytophthora capsici affecting chilli pepper (Capsicum annum L.). Scientific Reports, 10(1), 1–15. https://doi.org/10.1038/s41598-020-69410-3
Jha, Y., & Subramanian, RB (2012). Isolation of Root Associated Bacteria From the Local Variety of Rice Gj-17. World Research Journal of Geoinformatics, 1(2), 21–26.
Kang, SM, Khan, AL, Waqas, M., You, YH, Kim, JH, Kim, JG, Hamayun, M., & Lee, IJ (2014). Plant growth-promoting rhizobacteria reduce adverse effects of salinity and osmotic stress by regulating phytohormones and antioxidants in Cucumis sativus. Journal of Plant Interactions, 9(1), 673–682. https://doi.org/10.1080/17429145.2014.894587
Kaushal, J., Mehandia, S., Singh, G., Raina, A., & Arya, SK (2018). Catalase enzyme: Application in bioremediation and food industry. Biocatalysis and Agricultural Biotechnology, 16, 192–199. https://doi.org/10.1016/j.bcab.2018.07.035
Loks, N., Manggoel, W., Daar, J., Mamzing, D., & Seltim, B. (2014). The effects of fertilizer residues in soils and crop performance in northern Nigeria: A review. International Research Journal of Agricultural Science and Soil Science, 4(9), 180–184. http://interesjournals.org/full-articles/the-effects-of-fertilizer-residues-in-soils-and-crop-performance-in-northern-nigeria-a-review.pdf?view=inline
Manasa, K., Reddy, RS, & Triveni, S. (2017). Isolation and characterization of Pseudomonas fluorescens isolates from different rhizosphere soils of Telangana. Journal of Pharmacognosy and Phytochemistry, 6(3), 224–229.
Manikmas, MOA (2010). MERAUKE INTEGRATED RICE ESTATE ( MIRE ): RESURRECTION OF FOOD SECURITY AND INDEPENDENCE FROM THE EAST INDONESIAN CORPORATE Merauke Integrated Rice Estate ( MIRE ): The Awakening of Food Security and Food Sovereignty from The Eastern Part of Indonesia Going forward, keta. Agricultural Policy Analysis, 8(4), 323–338.
Mcdonald, G., Bovil, W., Lightfoot, DA, & Huang, C.-Y. (2013). Nutrient Use Efficiency. In Kole (Ed.), Genomics and Breeding for Climate-Resilient Crops: Vol. 2 (pp. 1–474). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-37048-9
Miller, SB, Heuberger, AL, Broeckling, CD, & Jahn, CE (2019). Non-targeted metabolomics reveals sorghum rhizosphere-associated exudates are influenced by the belowground interaction of substrate and sorghum genotype. International Journal of Molecular Sciences, 20(2). https://doi.org/10.3390/ijms20020431
Minorsky, PV (2019). On the inside. Plant Physiology, 179(4), 1431–1432. https://doi.org/10.1104/pp.19.00279
Mishra, D., Rajvir, S., Mishra, U., & Kumar, S. (2013). Role of Bio-Fertilizer in Organic Agriculture: A Review. Research Journal of Recent …, 2(2013), 39–41. http://isca.in/rjrs/archive/special_issue2012/8.ISCA-ISC-2012-1AFS-32.pdf
Modi, K., Patel, P., & Parmar, K. (2017). Isolation, Screening and Characterization of PGPR from Rhizosphere of Rice. International Journal of Pure & Applied Bioscience, 5(3), 264–270. https://doi.org/10.18782/2320-7051.2887
NING, C. chuan, GAO, P. dong, WANG, B. qing, LIN, W. peng, JIANG, N. hao, & CAI, K. zheng. (2017). Impacts of chemical fertilizer reduction and organic amendments supplementation on soil nutrients, enzyme activity and heavy metal content. Journal of Integrative Agriculture, 16(8), 1819–1831. https://doi.org/10.1016/S2095-3119(16)61476-4
Pambudi, A., Susanti, S., & Priambodo, TW (2017). Isolation and Characterization of Paddy Soil Bacteria in Sukawali Village and Belimbing Village, Tangerang Regency. Al-Kauniyah: Journal of Biology, 10(2), 105–113. https://doi.org/10.15408/kauniyah.v10i2.4907
Prasad, M., Srinivasan, R., Chaudhary, M., Choudhary, M., & Jat, LK (2019). Plant Growth Promoting Rhizobacteria (PGPR) for Sustainable Agriculture. In PGPR Amelioration in Sustainable Agriculture. Elsevier Inc. https://doi.org/10.1016/b978-0-12-815879-1.00007-0
Putri, AL (2017). Effect of fertilization application on population and diversity of actinomycetes from rhizosphere soils of Sorghum bicolor. In: International Proceedings of The 1st SATREPS Conference. Bogor November 14th, 2016, 1, 169–175.
Rai, A., Belkacem, M., Assadi, I., Bollinger, JC, Elfalleh, W., Assadi, AA, Amrane, A., & Mouni, L. (2022). Bacteria in Soil: Promising Bioremediation Agents in Arid and Semi-Arid Environments for Cereal Growth Enhancement. Applied Sciences (Switzerland), 12(22), 1–21. https://doi.org/10.3390/app122211567
Ray, P., Lakshmanan, V., Labbé, JL, & Craven, KD (2020). Microbe to Microbiome: A Paradigm Shift in the Application of Microorganisms for Sustainable Agriculture. Frontiers in Microbiology, 11(December), 1–15. https://doi.org/10.3389/fmicb.2020.622926
Roberts, DP, & Mattoo, AK (2019). Sustainable Crop Production Systems and Human Nutrition. Frontiers in Sustainable Food Systems, 3(September), 1–14. https://doi.org/10.3389/fsufs.2019.00072
Saha, ML, Afrin, S., Sony, SK, & Islam, MN (2017). Pigment Producing Soil Bacteria of Sundarban Mangrove Forest. Bangladesh Journal of Botany, 46(2), 717–724.
Santos-Medellín, C., Edwards, J., Liechty, Z., Nguyen, B., & Sundaresan, V. (2017). Drought Stress Results in a Compartment-Specific Restructuring of. MBio, 8(4:8:e00764-17), 1–15. http://www.ncbi.nlm.nih.gov/pubmed/28720730%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5516253
Sharma, A., Shankhdhar, D., Sharma, A., & Shankhdhar, SC (2014). Growth promotion of the rice genotypes by pgprs isolated from rice rhizosphere. Journal of Soil Science and Plant Nutrition, 14(2), 505–517. https://doi.org/10.4067/S0718-95162014005000040
Sherpa, MT, Sharma, L., Bag, N., & Das, S. (2021). Isolation, Characterization, and Evaluation of Native Rhizobacterial Consortia Developed From the Rhizosphere of Rice Grown in Organic State Sikkim, India, and Their Effect on Plant Growth. Frontiers in Microbiology, 12(September), 1–15. https://doi.org/10.3389/fmicb.2021.713660
Singh, JS, Pandey, VC, & Singh, DP (2011). Efficient soil microorganisms: A new dimension for sustainable agriculture and environmental development. Agriculture, Ecosystems and Environment, 140(3–4), 339–353. https://doi.org/10.1016/j.agee.2011.01.017
Sivojiene, D., Kacergius, A., Baksiene, E., Maseviciene, A., & Zickiene, L. (2021). The influence of organic fertilizers on the abundance of soil microorganism communities, agrochemical indicators, and yields in east Lithuanian light soils. Plants, 10(12). https://doi.org/10.3390/plants10122648
Tsukanova, KA, Сhеbоtаr, V., Meyer, JJM, & Bibikova, TN (2017). Effect of plant growth-promoting Rhizobacteria on plant hormone homeostasis. South African Journal of Botany, 113, 91–102. https://doi.org/10.1016/j.sajb.2017.07.007
Vacheron, J., Desbrosses, G., Bouffaud, ML, Touraine, B., Moënne-Loccoz, Y., Muller, D., Legendre, L., Wisniewski-Dyé, F., & Prigent-Combaret, C. (2013). Plant growth-promoting rhizobacteria and root system functioning. Frontiers in Plant Science, 4(SEP), 1–19. https://doi.org/10.3389/fpls.2013.00356
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., & Nasrulhaq Boyce, A. (2016). Role of plant growth promoting rhizobacteria in agricultural sustainability-A review. Molecules, 21(5), 1–17. https://doi.org/10.3390/molecules21050573
Wang, J., Liu, L., Gao, X., Hao, J., & Wang, M. (2021). Elucidating the effect of biofertilizers on bacterial diversity in maize rhizosphere soil. PLoS ONE, 16(April 4), 1–15. https://doi.org/10.1371/journal.pone.0249834
Wang, Y., Narayanan, M., Shi, X., Chen, X., Li, Z., Natarajan, D., & Ma, Y. (2022). Plant growth-promoting bacteria in metal-contaminated soil: Current perspectives on remediation mechanisms. Frontiers in Microbiology, 13(August), 1–17. https://doi.org/10.3389/fmicb.2022.966226
Wu, Z., Liu, Q., Li, Z., Cheng, W., Sun, J., Guo, Z., Li, Y., Zhou, J., Meng, D., Li, H., Lei, P., & Yin, H. (2018). Environmental factors shaping the diversity of bacterial communities that promote rice production. BMC Microbiology, 18(1), 1–11. https://doi.org/10.1186/s12866-018-1174-z
Xiang, W., Zhao, L., Xu, X., Qin, Y., & Yu, G. (2012). Mutual Information Flow between Beneficial Microorganisms and the Roots of Host Plants Determined the Bio-Functions of Biofertilizers. American Journal of Plant Sciences, 03(08), 1115–1120. https://doi.org/10.4236/ajps.2012.38134
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