Improving The Growth And Adaptation Of The Black Orchid Plantlet (Coelogyne Pandurata Lindl) In Various Growing Media by Giving Plant Extracts as Biostimulants at The Acclimatization Stage
Abstract
One of the most important stages in the process of transferring in vitro cultured seedlings to their natural environment is the acclimatization stage. The acclimatization stage is a critical period in plant propagation. The factors that most influence the growth of in vitro seedlings in the acclimatization process are suitable planting media and the administration of biostimulants. This study aimed to obtain the best planting medium and type of biostimulant for the growth of black orchid plantlets at the acclimatization stage. The study used a completely randomized design with a factorial pattern with two factors, namely first factor: type of growing media with 5 levels of treatment (wood sawdust, coconut coir, cocopeat, rice husk, sugarcane dregs) and second factor: type of biostimulant (without biostimulants; Moringa oleifera extract; Centella Asiatica extract; and Melastoma malabathricum extract). The results showed that the highest percentage of plantlet survival (100%) in the early stages of acclimatization was shown in plantlets grown on wood sawdust and coconut coir media. At the advanced acclimatization stage, the type of biostimulant significantly affected the parameters of survival percentage, tiller height, average number and width of leaves, and average number and length of roots. Optimal planting medium and biostimulant type for advanced acclimatization orchid growth is a single treatment of wood sawdust media with a tiller survival rate (100%), the highest number of leaves (4.65 strands), most roots (8.4 strands).
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Adi, N., Astarini, I., & Astiti, N. (2014). Aklimatisasi Anggrek Hitam (Coelogyne pandurata Lindl.) Hasil Perbanyakan In Vitro Pada Media Berbeda JurnalSimbiosis, II(2), 203–214.
https://ojs.unud.ac.id/index.php/simbiosis/article/view/9779
Andalasari, T. D., Yafisham, Y., & Nuraini, N. (2014). Respon Pertumbuhan Anggrek Dendrobium Terhadap Jenis Media Tanam dan Pupuk Daun. Pertanian Terapan, 14(3), 167–173. https://doi.org/10.25181/jppt.v14i3.156
Bulgari, R., Cocetta, G., Trivellini, A., Vernieri, P., & Ferrante, A. (2015). Biostimulants and crop responses: A review. Biological Agriculture and Horticulture, 31(1),1–17. https://doi.org/10.1080/01448765.2014.964649
Culver, M., Fanuel, T., & Chiteka, A. Z. (2012). Effect of Moringa Extract on Growth and Yield of Tomato. Greener Journal of Agricultural Sciences, 2(5), 207–211. https://doi.org/10.1501/eczfak_0000000528
du Jardin, P. (2015). Plant biostimulants:Definition, concept, main categories and regulation. In Scientia Horticulturae,196,3–14.Elsevier. https://doi.org/10.1016/j.scienta.2015.09.021
Ertani, A., Pizzeghello, D., Francioso, O., Tinti, A., & Nardi, S. (2016). Biological activity of vegetal extracts containing phenols on plant metabolism. Molecules, 21(2),1–14. https://doi.org/10.3390/molecules21020205
Febrianto, R., Suwirmen, & Syamsuardi. (2015). Aklimatisasi Planlet Kantong Semar (Nepenthes gracilis Korth.) pada berbagai Campuran Media Tanam Tanah Ultisol Jurnal Biologi Universitas Andalas, 4(2), 96–101.
Ginting. (2008). Membuat Media Tumbuh Anggrek. Surat Kabar Sinar Tani.
Hani, Zakiah, Z., & Mukarlina. (2022). The Acclimatization Stage Growth Of Black Orchid (Coelogyne Pandurata Lindl.) Plantlet By Biostimune Extract Gotu Kola (Centella Asiatica L .) Treatment. Jurnal Agronomi Tanaman Tropika, 4(1).
Hanik, N. R., Eskundari, R. D., & Wiharti, T. (2022). The Effect of Planting Media Composition on the Growth of Dendrobium Sp. Orchid Seedlings. Jurnal Biologi Tropis, 22(2), 485–493. https://doi.org/10.29303/jbt.v22i2.3446
Hariyanto, S., Jamil, A., & Purnobasuki, H. (2019). Effects of Plant Media And Fertilization on The Growth of Orchid Plant (Dendrobium sylvanum rchb. F.) in Acclimatiza tion Phase. Journal of Agro Sains, 7(1), 66–72. https://doi.org/10.18196/pt.2019.095.66-72
Karmila, R., Zakiah, Z., & Mukarlina. (2022). Aklimatization Black Orchid Plantlets (Coelogyne pandurata Lindl.) with Biostimulant Moringa Leaf Extract (Moringa oleifera Lamk.). Jurnal Biologi Tropis, 22(3), 954–961. https://doi.org/10.29303/jbt.v22i3.3593
Kasutjianingati, Firgiyanto, R., & Firnawati, L. (2020). The Growth of Vanda Seedling (Vanda sanderiana) with some Types of Plant Media Acclimatization and Concentration of Humic Acid. IOP Conference Series: Earth and Environmental Science, 411(1). https://doi.org/10.1088/1755-1315/411/1/012003
Kurniasih, W., Nabiila, A., Nurul Karimah, S., Farhan Fauzan, M., Riyanto, A., Rizal Putra, R., (2017). Pemanfaatan Batu Zeolit Sebagai Media Aklimatisasi Untuk Mengoptimalkan Pertumbuhan Anggrek Bulan (Phalaenopsis sp. ) Hibrida Bioma, 6(2), 29–41. https://doi.org/https://doi.org/10.26877/bioma.v6i2.1713
Nikmah, Z. C., Slamet, W., & Kristanto, B. A. (2017). Aplikasi silika dan NAA terhadap pertumbuhan Anggrek Bulan (Phalaenopsis amabilis l.) pada tahap aklimatisasi. Journal of Agro Complex, 1(3), 101–110. https://doi.org/10.14710/joac.1.3.101-110
Pichersky, E., & Raguso, R. A. (2018). Why do plants produce so many terpenoid compounds? In New Phytologist (Vol. 220, Issue 3, pp. 692–702). Blackwell Publishing Ltd. https://doi.org/10.1111/nph.14178
Rafiee, H., Naghdi Badi, H., Mehrafarin, A., Qaderi, A., Zarinpanjeh, N., Sekara, A., & Zand, E. (2016). Application of Plant Biostimulants as New Approach to Improve the Biological Responses of Medicinal Plants - A Critical Review. Journal of Medicinal Plants, 15(59), 6–39.
Singh, S., Mishra, S. P., Singh, P., & Prasad, R. S. (2013). Moringa Oleifera Leaf Extract As Biostimulant For Increasing Pea Yield. Indian Forester, 139(6), 562–563. http://www.indianforester.co.in.
Sukmadijaya, D., Dinarti, D., & Isnaini, Y. (2013). Pertumbuhan Planlet Kantong Semar (Nepenthes rafflesiana Jack.) pada Beberapa Media Tanam Selama Tahap Aklimatisasi. Jurnal Hortikultura Indonesia, 4(3),124. https://doi.org/10.29244/jhi.4.3.124-130
Zakiah, Z., Suliansyah, I., Bakhtiar, A., & Mansyurdin. (2017). Effect of Crude Extracts of Six Plants on Vegetative Growth of Soybean (Glycine max Merr.). International Journal of Advances in Agricultural Science and Technology, 4(7), 1–12. www.ijaast.com
Zi, J., Mafu, S., & Peters, R. J. (2014). To gibberellins and beyond! Surveying the evolution of (Di)terpenoid metabolism. In Annual Review of Plant Biology 65,259–286). Annual Reviews Inc. https://doi.org/10.1146/annurev-arplant-050213-035705
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