Isolation and Potential Test of Phosphate-Solubilizing Bacteria in the Rhizosphere of Mangrove Plants (Rhizophora mucronata Poir) as Isolates for Biofertilizer
Abstract
Mangrove forests play a significant role in environmental sustainability, particularly in their capacity to mitigate coastal erosion caused by seawater intrusion. Specifically, mangrove plants possess the ability to stabilize coastal land by retaining seawater, thereby preventing erosion along shorelines. This study aims to isolate phosphate-solubilizing bacteria and evaluate their potential from the rhizosphere of mangrove plants. The research was conducted at the Central Laboratory of the Stiper Agricultural Institute in Yogyakarta, located in Maguwoharjo, Depok District, Sleman Regency, Yogyakarta, with sampling carried out at Baros Beach in Kretek District, Bantul Regency, Yogyakarta. The study was conducted from January to February 2024. A descriptive research methodology was employed, including sterilizing tools and materials, media preparation, sampling, bacterial isolation, and identification. The analysis involved macroscopic and microscopic observations, measurement of the phosphate solubility index, and biochemical testing. The findings revealed a total of 16 bacterial isolates from five species of mangroves, specifically within the genera Streptococcus and Paracoccus. Notably, the isolate designated as SC 1 exhibited the highest phosphate solubility index, measuring 6.20 mm, indicating its potential for phosphate solubilization.
Downloads
References
Amin, S. S., Ghozali, T. Z., & Efendi, M. R. S. (2023). Identifikasi bakteri dari telapak tangan dengan pewarnaan Gram Identification of bacteria from palms with Gram stain. Jurnal Kimia dan Ilmu Lingkungan, 1(1), 30–35.
Anastia, N., & Lubis, S. S. (2022). Potential of phosphate solubilizing bacteria from mangrove rhizosphere effect plant growth corn (Zea mays). KENANGA Journal of Biological Science and Applied Biology, 2(1), 1–14. https://doi.org/10.22373/kenanga.v2i1.1919
Anggraini, I., Ferniah, R. S., & Kusdiyantini, E. (2019). Isolasi khamir fermentatif dari batang tanaman tebu (Saccharum officinarum L.) dan hasil identifikasinya berdasarkan sekuens internal transcribed spacer. Jurnal Berkala Bioteknologi, 2(2), 12–22.
Ariyani, A. N. (2020). Gambaran hasil uji hemolisis bakteri Streptococcus pyogenes yang diinokulasi pada media agar darah manusia kadaluarsa dan agar darah domba. (pp. 7–26).
Farid, E., & Setiadi. (2019). Teknik isolasi, karakterisasi, dan identifikasi bakteri pada ikan toman (Channa micropeltes). Jurnal Perikanan Indonesia, 17(2), 155–159.
Febriansah, R. E., & Meiliza, D. R. (2020). Buku ajar mata kuliah.
Hidayanti, A. S. N., Sulfiani, S., & Taufiq, N. (2021). Pemanfaatan ekstrak kulit ubi jalar ungu sebagai pengganti crystal violet pada pewarnaan Gram. Jurnal Sehat Mandiri, 16(2), 46–56. https://doi.org/10.33761/jsm.v16i2.364
Kristinanda, A. D. (2018). Isolasi bakteri pendegredasi senyawa hidrokarbon pada tanah tercemar di PT. KAI (Persero) UPT Balai Yasa Yogyakarta. Universitas Islam Indonesia.
Lestari, W., Linda, M., & Martina, A. (2011). Kemampuan bakteri pelarut fosfat isolat asal Sei Garo dalam penyediaan fosfat terlarut dan serapannya pada tanaman kedelai (Capability of phosphate solubilizing bacteria from Sei Garo in soluble phosphate and its uptake by soybean). Biospecies, 4(2), 15.
Mardyansah, D., & Trimulyono, G. (2021). Isolasi, karakterisasi, dan uji potensi bakteri pelarut fosfat dari rhizosfer di Pegunungan Kapur Selatan, Tulungagung. LenteraBio: Berkala Ilmiah Biologi, 10(2), 188–198. https://doi.org/10.26740/lenterabio.v10n2.p188-198
Marista, E., Khotimah, S., & Linda, R. (2013). Bakteri pelarut fosfat hasil isolasi dari tiga jenis tanah rizosfer tanaman pisang nipah (Musa paradisiaca var. nipah) di Kota Singkawang. Protobiont, 2(2), 93–101.
Maudy, R. N., Zulaika, E., & Shovitri, M. (2020). Karakter isolat bakteri P1 dari rhizosfer tanaman tebu (Saccharum officinarum). Jurnal Sains dan Seni ITS, 8(2), 2–3. https://doi.org/10.12962/j23373520.v8i2.43550
Nuryanti, S., & Pratiwi, A. R. (2021). Karakterisasi isolat bakteri penghasil selulosa dari buah naga merah (Hylocereus polyrhizus). Jurnal Teknologi Pangan Indonesia, 13(1), 71–79.
Oksana, O., Irfan, M., Fianiray, A. R., & Zam, S. I. (2020). Isolasi dan identifikasi bakteri pelarut fosfat pada tanah ultisol di Kecamatan Rumbai, Pekanbaru. Agrotechnology Research Journal, 4(1), 22–25. https://doi.org/10.20961/agrotechresj.v4i1.36063
Pane, R. D. P., Ginting, E. N., & Hidayat, F. (2022). Mikroba pelarut fosfat dan potensinya dalam meningkatkan pertumbuhan tanaman. Warta PPKS, 27(1), 51–59.
Rifai, K. R. (2021). Uji indole sebagai kegiatan penjaminan mutu tambahan pada hasil pengujian coliform dalam sampel air mineral. Jurnal Teknologi Proses dan Inovasi Industri, 6(1), 1–6.
Sharon, J. A., Hathwaik, L. T., Glenn, G. M., Imam, S. H., & Lee, C. C. (2016). Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth. Journal of Soil Science and Plant Nutrition, 16(2), 525–536. https://doi.org/10.4067/S0718-95162016005000043
Sianipar, G. W. S., Sartini, S., & Riyanto, R. (2020). Isolasi dan karakteristik bakteri endofit pada akar pepaya (Carica papaya L.). Jurnal Ilmiah Biologi UMA, 2(2), 83–92. https://doi.org/10.31289/jibioma.v2i2.312
Surtiningsih, T. (2015). Peran biofertilizer dari campuran mikroorganisme sebagai upaya untuk meningkatkan produktivitas tanaman pangan nasional. Perpustakaan Universitas Airlangga, 3(2), 123–129.
Syah, R. F., Irianto, A., & Ratnaningtyas, N. I. (2018). Biodegradation of diesel oil by yeast isolated from mangrove’s rhizosphere. Scripta Biologica, 5(2), 79–82.
Syarwani, A., Aisyah, S., Hadija, & Nirawati. (2022). Isolasi dan identifikasi bakteri pelarut fosfat dari rhizosfer tanaman aren (Arenga pinnata (Wurmb) Merr) di Desa Bonto Somba Kabupaten Maros. Eboni, 4(2), 63–70.
Copyright (c) 2025 Nadhifa Zahra Alhafiza, Ryan Firman Syah, Elisabeth Nanik Kristalisasi
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Jurnal Agronomi Tanaman Tropika (JUATIKA) agree to the following terms:
Authors retain copyright and grant the Jurnal Agronomi Tanaman Tropika (JUATIKA) right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0) that allows others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, even commercially) with an acknowledgment of the work's authorship and initial publication in Jurnal Agronomi Tanaman Tropika (JUATIKA).
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Agronomi Tanaman Tropika (JUATIKA). Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
More Information
