Potential Antioxidant Content of Three Types of Mimosa Weed from Various Plant Parts using The DDPH (2,2-diphenyl-1-picrylhydrazyl) Method
Abstract
Antioxidants are essential for neutralizing free radical and their presence in plants like mimosa weeds could be beneficial for various applications, include medical uses and agricultural development. This research focus on the potential antioxidant content of mimosa weed species (Mimosa pudica L., Mimosa invisa L., Mimosa pigra L.) from various plant parts (roots, stems, leaves and seed) using the DPPH method (2,2-diphenyl-1-picrylhydrazyl). This study aims to understand the interaction between different types of mimosa weeds and various plant parts in relation to antioxidant activity levels, as well as phytochemical content across the three types of Mimosa sp. using the DPPH method and determine the best-performing weed and plant parts based on the smallest IC50 value and highest phytochemical content. This research was conducted in Padang City. Sample drying and extraction were carried out at the Plant Physiology Laboratory, Faculty of Agriculture, Andalas University, and phytochemical tests were performed at Vahana Scientific Laboratory Padang. The research took place from February to May 2024. The design used was a factorial Completely Randomize Design (CRD). The first factor is the treatment of Mimosa sp. weed species, namely: Mimosa pudica L., Mimosa invisa L. and Mimosa pigra L. The second factor was the treatment of different parts of the Mimosa sp. Namely: root, stem, leaf and seed. From these two factors, 12 treatment combinations were obtained, each repeated three times, resulting in a total of 36 experimental units. The research data were analyzed statistically with the F test at the 5% level and if F count > F table at 5%, then it continued with Duncan’s New Multiple Range Test (DNMRT) at the 5% level. The results showed that there was an interaction between mimosa weed species and plant parts in terms of antioxidant activity, as indicated by the IC50 values. The strongest antioxidant was found in the leaves of Mimosa pigra L., with an antioxidant value of 41.89 mg/L.
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