The Acclimatization Stage Growth Of Black Orchid (Coelogyne Pandurata Lindl.) Plantlet By Biostimune Extract Gotu Kola (Centella Asiatica L.) Treatment
Abstract
Black orchid plantlets have a relatively slow growth at the acclimatization stage. The growth of black orchid plantlets can be increased by giving biostimulants. Biostimulants are bioactive compounds, and when they are applied, they can increase plant growth. This study aims to determine the effect of biostimulant Gotu kola extract (Centella Asiatica L.) on the growth of black orchid plantlets (Coelogyne Pandurata Lindl.) at the acclimatization stage. The research was conducted at the Biology Department's greenhouse from September until December 2020. The study used a completely randomized design with a factorial pattern. The first factor is the concentration of biostimulants consisting of 5 levels, e.g: 0 mg/L; 25 mg/L; 50 mg/L; 75 mg/L; 100 mg/L. The second factor is the application time which consists of 2 levels: once a week and once every two weeks. The results showed that the application of biostimulant Gotu kola extract had no significant effect on plantlet height, leaf length, leaf width, number of leaves, number of tillers, and number of plantlet roots of black orchid in the acclimatization stage.
References
Adi, N., Astarini, I., & Astiti, N. (2014). Aklimatisasi Anggrek Hitam (Coelogyne pandurata Lindl.) Hasil Perbanyakan In Vitro pada Media Berbeda. Jurnal Simbiosis, 2(2), 203–214.
Agustin, A., Riniarti, M., & Duryat. (2014). Pemanfaatan Limbah Serbuk Gergaji dan Arang Sekam Padi sebagai Media Sapih untuk Cempaka Kuning (Michelia champacea). Jurnal Sylva Lestari, 2(3), 49–58.
Aulya, N., Noli, Z., Bakhtiar, A., & Mansyurdin. (2018). Effect of Plant Extracts on Growth and Yield of Maize (Zea mays L.). Pertanika J. Trop. Agric. Sc, 41(3), 1193–1205.
Bhattacharyya, P., Ghosh, S., Mandi, S., Kumaria, S., & Tandon, P. (2017). Genetic Variability and Association of AFLP Markers with Some Important Biochemical Traits in Dendrobium thyrsiflorum threatened Medicinal Orchid. S. Afr. J. Bot, 109, 214–222.
Bulgari, R., Franzoni, G., & Ferrante, A. (2019). Biostimulants Application in Horticultural Crops under Abiotic Stress Condition. Journal of Agronomy, 9(306), 1–30.
Drew, A., Kavanagh, K., & Maynard, C. (1992). Acclimatizing Micropropagated Black Cherry by Comparison with Half-Sib Seedlings. Physiol Plant, 86. 459–464.
Du Jardin, P. (2015). Plant Biostimulants: Definition, concept, main categories, and regulation. Scientia Horticulturae, 196. 314.
Handini, E. (2019). Penyimpanan biji dan mikropropagasi anggrek pensil (Papilionanthe hookeriana) di Kebun Raya Bogor. Pros Sem Nas Masy Biodiv Indon, 5(1), 7–12.
Hardjo, P. H. (2018). Kultur Jaringan Anggrek: Embriogenesis somatik Vanda tricolor (Lindl.) var. Pallida. Graha Ilmu.
Hartati, S., Nandariyah, Yunus, A., & Djoar, W. (2017). Short Communication: Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley). BIODIVERSITAS, 18(2), 555–559.
Hazarika, B. (2006). Morpho-physiological Disorders in In Vitro Culture of Plants. Sci Hort, 108, 105–120.
Hidayat, S., Saputri, W., & Astriani, M. (2018). Metodologi Penelitian Biologi. Universitas Muhammadiyah Palembang Press.
Irsyadi, M. B. (2021). Factors That Effect of the Optimal Plantlet Growth from Tissue Culture on the Acclimatization Stage. Proc. Internat. Conf. Sci. Engin, 4. 100–104.
Izudin, E. (2013). Teknik Aklimatisasi Tanaman Hasil Kultur Jaringan. Jurnal Informasi Teknis, 11(2), 49–56.
Konar, S., Karmakar, J., Ray, A., Adhikari, S., & Bandyopadhhyay, T. (2018). Regeneration of Plantlets Through Somatic Embryogenesis from Root Derived Calli of Hibiscus sabdariffa L. (Roselle) and Assessment of Genetic Stability by Flow Cytometry and ISSR Analysis. PLoS One, 13.
Kustini, S. J. (2011). Factsheet Anggrek Hitam. Yayasan WWF Indonesia.
Lakitan, B. (1995). Fisiologi Pertumbuhan dan Perkembangan Tanaman. Raja Grafindo Persada.
Lindsey, K., Jager, A., & van Steden, J. (1998). Effect of A Seaweed Concentrate on Acclimatization of In Vitro Grown Planlets of Kniphofia pauciflora and Scilla krausii. S Afr J Bot, 64(4), 262–264.
Managanta, A., & Pangli, M. (2014). Studi Habitat dan Inventarisasi Anggrek Hitam (Black Orchid) di Kawasan Hutan Cagar Alam Bancea Kecamatan Pamona Selatan, Kabupaten Poso. Jurnal Agro Pet, 11(1), 19–45.
Nawawi. (2014). Jenis-Jenis Anggrek Epifit pada Kawasan Hutan Bremi Distrik Manokwari Utara. Jurnal Biodiversitas, 1(2), 7–10.
Nugroho, C., & Raden, I. (2021). Aklimatisasi Tiga Jenis Anggrek pada Media Tanam yang Berbeda. Jurnal Pertanian, 12(2), 109–107.
Paradikovic, N., Teklic, T., Zeljkovic, S., Lisjak, M., & Spoljarevic, M. (2018). Biostimulants Research in Some Horticultural Plant Species. Food and Energy Security, 1–17.
Prasetyorini. (2019). Kultur Jaringan (1st ed.). Lembaga Penelitian dan Pengabdian Masyarakat Universitas Pakuan.
Rafiee, H., Naghdi, B., Mehrafarin, A., Qaderi, A., Zarinpanjeh, N., Sekara, A., & Zand, E. (2016). Application of Plant Biostimulants as New Approach to Improve the Biological Responses of Medicinal Plants. Journal of Medicinal Plants, 15(59), 6–39.
Ramadanil. (2010). Beberapa Aspek Botani Anggrek Endemik Coelogyne celebensis J.J. Sm dari Taman Nasional Lore Lindu Sulawesi Tengah. Jurnal Biocelebes, 4(1), 1–14.
Sandra, E. (2005). Membuat Anggrek Rajin Berbunga. Agromedia Pustaka.
Silva, J., Hossain, M., Sharma, M., Dobranszki, J., Cardoso, J., & Songjun, Z. (2017). Acclimatization of in Vitro-derived Dendrobium. Horticultural Plant Journal, 3(3), 110–124.
Silva, S., Brown, P., & Ponchet, M. (2013). First World Congress on the Use of Biostimulants in Agriculture. International Society of Horticultural Science.
Sri, G. (2010). Pengaruh Ekstrak Pegagan (Centella asiatica (L.) Urban) sebagai Alelokemi terhadap Perkecambahan dan Pertumbuhan Bayam Duri (Amaranthus spinosus) serta Tomat (Lycopersicum esculentum) [Skripsi]. Univrsitas Sebelas Maret.
Ummah, K., Noli, Z., Bakhtiar, A., & Mansyurdin. (2017). Effect of Certain Plant Crude Extracts on the Growth of Upland Rice (Oryza sativa L.). Int. J. Curr. Res. Biosci. Plant Biol, 4(9), 1–6.
Untari, R., Sandra, E., & Puspitaningtyas, D. (2007). Aklimatisasi Bibit Anggrek Hitam (Coelogyne pandurata Lindl.). Buletin Kebun Raya Indonesia, 10(1), 13–19.
Van Oosten, M., Pepe, O., Pascale, S., Silletti, S., & Maggio, A. (2017). The Role of Biostimulants and Bioeffectors as Alleviators of Abiotic Stress in Crop Plants. Chem Biol Technol Agric, 4(5), 1–12.
Wahyuni, H., & Suranto. (2021). Dampak Deforestasi Hutan Skala Besar terhadap Pemanasan Global di Indonesia. Jurnal Ilmiah Ilmu Pemerintahan, 6(1), 148–162.
Waluya, A. (2009). Aklimatisasi Planlet Hasil Perbanyakan secara Kultur Jaringan. Departemen Agronomi dan Hortikultura Institut Pertanian Bogor.
Zakiah, Z., Suliansyah, I., Bakhtiar, A., & Mansurdin. (2017). Effect of Crude Extracts of Six Plants on Vegetative Growth of Soybean (Glycine max Merr.). Journal of Advances in Agricultural Science an Technology, 4(7), 1–12.
Ziv, M., & Chen, J. (2008). The Anatomy and Morphology of Tissue Cultured Plants. Springer, 1(3), 465–477.
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