A Test for Bacillus Spp. Ability to Suppress Pestalotiopsis sp. Causing Rubber Leaf Drought Through In-Vitro
Abstract
Using synthetic chemical pesticides can cause environmental pollution, the death of biological agents, the emergence of residues, and is dangerous for humans. One environmentally friendly alternative for disease control is biological control using Bacillus spp. This study aimed to test the antagonistic ability of Bacillus spp., to obtain a morphological description of Pestalotiopsis sp. and get a type of Bacillus bacteria that can inhibit the fungus Pestalotiopsis sp. causes of rubber leaf fall disease in vitro. This research was carried out at the Plant Disease Laboratory, Faculty of Agriculture, Riau University and the Pekanbaru Class 1 Quarantine Laboratory. This research was carried out experimentally using a completely randomized design (CRD) consisting of 7 treatments and 4 replications. Bacillus spp that used namely (B0) Without Bacillus spp. (B1) Bacillus amyloliquefaciens, (B2) Bacillus cereus, (B3) Bacillus pseudomycoides, (B4) Bacillus velezensis 1, (B5) Bacillus velezensis 2, (B6) Bacillus velezensis 3. The data obtained from the research results were analyzed statistically using variance analysis and further tested using the DNMRT Test at the 5% level. All Bacillus spp. are able to inhibit the fungus Pestalotiopsis sp., but 2 Bacillus, namely B. cereus B. pseudomycoides, are not able to produce an inhibition zone. B. amyloliquefaciens is a type of Bacillus that is more capable of inhibiting the fungus Pestalotiopsis sp. with an inhibitory power of 69.85% and an inhibitory zone of 64.74% and can reduce the length and width of conidia.References
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