Response Growth of Explant Organs Seed of Red Dragon Fruit (Hylocerus polyrhizus) On MS Media (Murashige and Skoog) by Adding Kinetin And Sucrose
Abstract
Dragon fruit, also known as pitaya, is a plant that originates from Central and South America and is classified as a type of cactus. It belongs to the genus Hylocereus and the family Cactaceae. This research investigates the growth response of explant organs from red dragon fruit (Hylocereus polyrhizus) on Murashige and Skoog (MS) media by adding kinetin and sucrose. The study was conducted in the UPT Seed Network Plant Food Horticulture Laboratory in Riau Province, located on Kaharudin Nasution Road, Crossroads Three Sub-district, Bukit Raya District, Pekanbaru City, for 4 months. This research This Design Random Complete (RAL) Factorial consists of 2 level treatment with three repetitions. The results of the study indicate that the concentrations of Kinetin and Sucrose have a significant impact on the observed parameters. The treatment with kinetin yielded the best results for the number of shoots, shoot height, and thorns compared to the control treatment without kinetin. Specifically, the average results were 1.47 shoots, a shoot height of 1.65 cm, and 16.83 thorns in the control group. In contrast, the treatment with 5 mg/L Kinetin produced an average of 3.50 shoots, while the treatment with 2.5 mg/L Kinetin resulted in a root length of 6.88 cm. For the Sucrose treatment, the optimal concentration was found to be 60 g/L, which resulted in an average of 1.42 shoots, a shoot height of 1.18 cm, and a root length of 7.64 cm. The treatment with 40 g/L Sucrose yielded an average of 10.94 thorns and 3.69 roots. The study also revealed a significant interaction between Kinetin and Sucrose, particularly affecting the number of thorns. The treatment with 2.5 mg/L Kinetin and 40 g/L Sucrose resulted in an average of 14.22 thorns, while the combination of 2.5 mg/L Kinetin and 20 g/L Sucrose produced an average root length of 8.65 cm. These findings provide valuable insights into the effects of Kinetin and Sucrose on the growth parameters of Hylocerus Dragon Fruit (Hylocereus polarizes).
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Aulia, N. H., & Setyorini, T.* (2023). Perbanyakan dan pembentukan umbi mikro kentang (Solanum tuberosum L.) secara in vitro pada modifikasi komposisi media MS dan sukrosa. Jurnal Agrin, 27(1).
Ernayunita, E., Rahmadi, H. Y., Harahap, I. Y., & Purba, A. R. (2016). The role of NAA, GA3, activated charcoal, and sucrose on zygotic embryos culture of hybrid OG open pollinated clone (Elaeis guineensis Jacq. × Elaeis oleifera). Jurnal Penelitian Kelapa Sawit, 24(3), 115–126. https://doi.org/10.22302/iopri.jur.jpks.v24i3.15
Fatonah, S., Novaliza, A., Isda, M., & Lestari, W. (2016). Induksi tunas in vitro jeruk siam (Citrus nobilis Lour.) asal Kampar pada berbagai konsentrasi sukrosa. Jurnal Riau Biologia, 1(1), 80–85. https://scholar.google.com/citations?view_op=view_citation&hl=en&user=HieaTioAAAAJ&pagesize=80&citation_for_view=HieaTioAAAAJ:35r97b3x0nAC
Finna, R. L., & Mukarlina. (2015). Pertumbuhan secara in vitro tunas buah naga merah (Hylocereus polyrhizus [Weber] Britton & Rose) dengan penambahan naftalene acetic acid (NAA) dan air kelapa. Jurnal Protobiont, 4(3), 103–108.
Hapsari, B. W., Martin, A. F., & Ermayanti, T. M. (2015). Pengaruh konsentrasi gula terhadap pertumbuhan kultur tunas Tacca leontopetaloides. Prosiding Seminar Nasional XVIII “Kimia Dalam Pembangunan”, September, 227–232. https://doi.org/10.13140/RG.2.1.4566.3760
Heriansyah, P. (2019). Multiplikasi embrio somatis tanaman anggrek (Dendrobium sp.) dengan pemberian kinetin dan sukrosa secara in vitro. Jurnal Ilmiah Pertanian, 15(2), 67–78. https://doi.org/10.31849/jip.v15i2.1974
Inda Hidayati, R., Rahayu, A., & Sulassih. (2021). Perbanyakan tanaman nilam (Pogostemon cablin Benth.) menggunakan sistem fotoautotrofik dengan berbagai konsentrasi gula dan jumlah ventilasi. Jurnal Agronomi Indonesia, 49(2), 212–218. https://doi.org/10.24831/jai.v49i2.35576
Istiqhomah, S., Mukaromah, A. S., & Rusmadi, R. (2019). Pengaruh kepadatan medium MS0 terhadap perkecambahan biji jagung (Zea mays L., var.”Lokal”) secara in vitro. Al-Hayat: Journal of Biology and Applied Biology, 2(2), 68. https://doi.org/10.21580/ah.v2i2.4664
Kadafi, M., & Marlina, E. I. (2023). Respon pertumbuhan eksplan jeruk siam (Citrus nobilis L.) terhadap pemberian hormon NAA dan kinetin pada media MS. Jurnal Green Swarnadwipa, 12(1), 183–191. https://doi.org/10.14341/diaconfiii25-26.05.23-62
Kalve, S., De Vos, D., & Beemster, G. T. S. (2014). Leaf development: A cellular perspective. Frontiers in Plant Science, 5. https://doi.org/10.3389/fpls.2014.00362
Karimah, N., Kusmiyati, F., & Anwar, S. (2021). Pengaruh penggunaan sukrosa dan IBA terhadap induksi akar eksplan tunas anggrek (Dendrobium sp.) secara in vitro. Jurnal Agrotek, 5(1), 34–44. https://jurnal.fp.umi.ac.id/index.php/agrotek/article/view/158
Khaerasani, I., Prihastanti, E., & Haryanti, S. (2017). Pertumbuhan kalus eksplan rimpang jahe merah (Zingiber officinale Rosc.) pada berbagai konsentrasi sukrosa secara in vitro. Buletin Anatomi dan Fisiologi, 2(1), 43. https://doi.org/10.14710/baf.2.1.2017.43-49
Mahadi, I. (2016). Effect of naftalen acetyl acyd (NAA) and kinetin hormones on tissue culture of Bogor pineapple (Ananas comosus (L.) Merr.) cv. Queen. Program Studi Pendidikan Biologi. Fakultas Keguruan dan Ilmu.
Miswan, M., Said, I., & Ratman, R. (2018). Penentuan kadar kalsium (Ca) dalam kulit dan daging buah naga merah (Hylocereus polyrhizus) yang berasal dari Sidera. Jurnal Akademika Kimia, 7(3), 136. https://doi.org/10.22487/j24775185.2018.v7.i3.11909
Mustikawaty, P. N., & Saputro, N. W. (2021). Induction of apple cucumber (Cucumis melo) buds development by combination of kinetin and IAA in B5 (Gamborg) medium. Mangifera Edu, 6(1), 44–55. https://doi.org/10.31943/mangiferaedu.v6i1.114
Ni’mah, F., Ratnasari, E., & Budipramana, L. S. (2012). Pengaruh pemberian berbagai kombinasi konsentrasi sukrosa dan kinetin terhadap induksi umbi mikro kentang (Solanum tuberosum L.) kultivar Granola Kembang secara in vitro. Jurnal Lenterabio, 1(1), 41–48. https://core.ac.uk/download/pdf/230674838.pdf
Nugroho, D. S. (2015). Pengaruh pemberian auksin (NAA) dengan sitokinin (BAP, kinetin, dan 2ip) terhadap daya proliferasi tanaman kantong semar (Nepenthes mirabilis) secara in vitro. Bul. Agrohorti, 151(3), 10–17.
Rakhmadhan Niah, H. (2016). Aktivitas antioksidan ekstrak etanol kulit buah naga merah daerah Pelaihari, Kalimantan Selatan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Jurnal Pharmascience, 3(2), 36–42.
Sudarjat, Leovika, A., Suminar, E., Isnaniawar, V., Abdilah Ha, M., Albi Fauzi, A., & Mubarok, S. (2018). Morphological characterization and adaptation of four dragon fruit genotypes in Pangandaran Regency of Indonesia. Asian Journal of Plant Sciences, 18(1), 21–25. https://doi.org/10.3923/ajps.2019.21.25
Wahidah, S. R. I. (2011). Pengaruh hormon kinetin terhadap pertumbuhan kalus rumput laut Kappaphycus alvarezii melalui kultur in vitro. Jurnal Vokasi, 7(2), 192–197.
Wijayanti, I., Novaliza Isda, M., Lestari, W., Program, M., Biologi, S., Botani, D. B., Biologi, J., Matematika, F., Ilmu, D., & Alam, P. (2015). Induksi akar jeruk siam asal Kampar (Citrus nobilis Lour.) dari tunas in vitro dengan berbagai kombinasi sukrosa dan NAA pada media ½ Murashige and Skoog. JOM FMIPA Volume, 2(1), 144–152.
Wulannanda, A., Anwar, S., & Kusmiyati, F. (2023). Kajian penambahan kinetin dan 2,4-D terhadap pertumbuhan kultur jaringan tanaman pisang Barangan (Musa paradisiaca L.) pada fase subkultur. Agroteknika, 6(1), 1–12. https://doi.org/10.55043/agroteknika.v6i1.161
Yasmin, Z. F., Aisyah, S. I., & Sukma, D. (2018). Pembibitan (kultur jaringan hingga pembesaran) anggrek Phalaenopsis di Hasanudin Orchids, Jawa Timur. Buletin Agrohorti, 6(3), 430–439. https://doi.org/10.29244/agrob.v6i3.21113
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