The Effect of Shelf Life and the Effectiveness of Biopesticide Fobio in Controlling Stem Rot Disease in Vanilla Plants (Vanilla planifolia Andrews)
Abstract
Stem rot disease caused by Fusarium oxysporum poses a significant challenge to vanilla (Vanilla planifolia
Andrews) cultivation. The use of microbial-based biopesticides, such as FOBIO, provides an environmentally
friendly alternative to reduce reliance on chemical fungicides. This study aimed to evaluate the effects of FOBIO
biopesticide storage age and concentration on disease intensity and vanilla plant growth (Vanilla planifolia
Andrews). The research was conducted from March to June 2025 in Dusun Tukum using a factorial completely
randomized design with two factors: FOBIO storage age (6 months and 1 year) and concentrations (5, 7.5, and 10
mL/L of water), with three replications totaling 105 plants. Data were analyzed using Analysis of Covariance
(ANCOVA), followed by the Least Significant Difference (LSD) test at a 5% significance level. The results
demonstrated that the FOBIO application significantly reduced disease intensity compared to the control (p <
0.05). The most effective treatment was FOBIO stored for 1 year at a concentration of 7.5 mL/L of water (FP23B),
resulting in a final disease intensity of 6.365 compared to 38.255 in the control. FOBIO also increased leaf number
and stimulated plant growth during the vegetative phase, including accelerating flower development. The FOBIO
biopesticide is an environmentally friendly alternative for controlling stem rot in vanilla and serves as a biological
control agent with potential to support sustainable agriculture.
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