Response of Four Lettuce Varieties to the NFT Hydroponic System with 3% H2O2 Application

  • Rennanti Lunnadiyah Aprilia Universitas Ma'arif Nahdlatul Ulama Kebumen
  • Alfi Nur Laila Universitas Ma'arif Nahdlatul Ulama Kebumen
Keywords: H2O2, Lactuca sativa, NFT Hydroponics, Root Health, Varietal Response

Abstract

Root health disorders are among the primary constraints in hydroponic lettuce cultivation, especially under conditions of suboptimal aeration, high temperatures, and fluctuating humidity. Hydrogen peroxide (H2O2) is commonly used to increase dissolved oxygen availability, suppress certain pathogens, and promote root health. This study aimed to evaluate the response of four lettuce varieties grown in a Nutrient Film Technique (NFT) hydroponic system with the application of 3% H2O2 and to identify the variety with the best agronomic performance. The research was conducted from October to December 2023 at Kebun Sayur 24 Hidroponik, Bojongsari Village, Alian District, Kebumen Regency. The experiment was arranged in a nonfactorial completely randomized design with four varieties—RZ Junction, Romaine, Mondai, and Rex—each replicated six times. The observed variables included plant height, root length, number of leaves, fresh plant weight, dry plant weight, fresh root weight, dry root weight, and leaf area at harvest, 45 days after sowing. Data were analyzed using analysis of variance followed by the 5% Honestly Significant Difference test. The results showed that varietal differences had a highly significant effect on plant height, number of leaves, dry plant weight, fresh root weight, dry root weight, and leaf area, but had no significant effect on root length and fresh plant weight. Romaine showed the best performance in plant height (14.37 cm), fresh root weight (9.17 g), dry root weight (0.66 g), and leaf area (671 cm²), whereas Rex produced the highest number of leaves (15.50). Therefore, Romaine was the most promising variety for NFT hydroponic lettuce cultivation under 3% H2O2 application.

Downloads

Download data is not yet available.

References

Ahmed, Z. F. R., Alnuaimi, A. K. H., Askri, A., & Tzortzakis, N. (2021). Evaluation of lettuce (Lactuca sativa L.) production under hydroponic system: Nutrient solution derived from fish waste vs. inorganic nutrient solution. Horticulturae, 7(9), 292. https://doi.org/10.3390/horticulturae7090292

Aprilia, R. L., & Fatmawati, N. (2020). Pengaruh teknik budidaya alternatif menggunakan zone cooling terhadap pertumbuhan dan hasil benih kentang di Desa Karangmangu Kecamatan Baturaden. Jurnal Kridatama Sains dan Teknologi, 2(2), 141–148. https://doi.org/10.53863/kst.v2i02.123

Baiyin, B., Tagawa, K., Yamada, M., Wang, X., Yamada, S., Yamamoto, S., & Ibaraki, Y. (2021). Study on plant growth and nutrient uptake under different aeration intensity in hydroponics with the application of particle image velocimetry. Agriculture, 11(11), 1140. https://doi.org/10.3390/agriculture11111140

Balliu, A., Zheng, Y., Sallaku, G., Fernández, J. A., Gruda, N. S., & Tuzel, Y. (2021). Environmental and cultivation factors affect the morphology, architecture and performance of root systems in soilless grown plants. Horticulturae, 7(8), 243. https://doi.org/10.3390/horticulturae7080243

Bello, N. M., & Renter, D. G. (2018). Invited review: Reproducible research from noisy data: Revisiting key statistical principles for the animal sciences. Journal of Dairy Science, 101(7), 5679–5701. https://doi.org/10.3168/jds.2017-13978

Hendrickson, T. D., Dunn, B. L., Goad, C., Hu, B., & Singh, H. (2022). Effects of hydrogen peroxide products on basil, lettuce, and algae in an ebb and flow hydroponic system. Horticulturae, 8(7), 569. https://doi.org/10.3390/horticulturae8070569

Hooks, T., Sun, L., Kong, Y., Masabni, J., & Niu, G. (2022). Short-term pre-harvest supplemental lighting with different light emitting diodes improves greenhouse lettuce quality. Horticulturae, 8(5), 435. https://doi.org/10.3390/horticulturae8050435

Khodijah, N. S., & Kusmiadi, R. (2021). The growth of lettuce (Lactuca sativa) hydroponically in a simple wick system on various types of nutrient composition. Jurnal Agronomi Tanaman Tropika (Juatika), 3(2), 180–186. https://doi.org/10.36378/juatika.v3i2.1366

Lau, V., & Mattson, N. (2021). Effects of hydrogen peroxide on organically fertilized hydroponic lettuce (Lactuca sativa L.). Horticulturae, 7(5), 106. https://doi.org/10.3390/horticulturae7050106

Marini, R. P. (2024). Experimental designs and statistical analyses for rootstock trials. Agronomy, 14(10), 2312. https://doi.org/10.3390/agronomy14102312

Matysiak, B., Kaniszewski, S., & Mieszczakowska-Frąc, M. (2023). Growth and quality of leaf and romaine lettuce grown on a vertical farm in an aquaponics system: Results of farm research. Agriculture, 13(4), 897. https://doi.org/10.3390/agriculture13040897

Nguyen, C. D., Creech, M., Xiang, D., Sandoya, G., Kopsell, D., & Huo, H. (2022). Performance of different lettuce cultivars grown hydroponically under fluorescent and light-emitting diode light growth conditions. HortScience, 57(11), 1447–1452. https://doi.org/10.21273/HORTSCI16780-22

Nitu, O. A., Ivan, E. Ș., Tronac, A. S., & Arshad, A. (2024). Optimizing lettuce growth in nutrient film technique hydroponics: Evaluating the impact of elevated oxygen concentrations in the root zone under LED illumination. Agronomy, 14(9), 1896. https://doi.org/10.3390/agronomy14091896

Rohman, F., & Aprilia, R. L. (2025). Kajian pertumbuhan dan hasil berbagai varietas selada (Lactuca sativa) menggunakan teknik aquaponik pada kolam ikan nila di Desa Pesantren, Wanayasa, Banjarnegara. Nusantara Hasana Journal, 5(1), 189–195. https://doi.org/10.59003/nhj.v5i1.1486

Sapkota, S., Sapkota, S., & Liu, Z. (2019). Effects of nutrient composition and lettuce cultivar on crop production in hydroponic culture. Horticulturae, 5(4), 72. https://doi.org/10.3390/horticulturae5040072

Sublett, W. L., Barickman, T. C., & Sams, C. E. (2018). The effect of environment and nutrients on hydroponic lettuce yield, quality, and phytonutrients. Horticulturae, 4(4), 48. https://doi.org/10.3390/horticulturae4040048

Thakulla, D., Dunn, B., Hu, B., Goad, C., & Maness, N. (2021). Nutrient solution temperature affects growth and °Brix parameters of seventeen lettuce cultivars grown in an NFT hydroponic system. Horticulturae, 7(9), 321. https://doi.org/10.3390/horticulturae7090321

Wang, W., Lin, Z., Wang, W., Shang, M., Lv, H., Zong, Q., Li, J., Liang, B., & Zhou, W. (2023). Elicitation with hydrogen peroxide promotes growth, phenolic enrichment, antioxidant activity and nutritional values of two hydroponic lettuce genotypes. Food Chemistry: X, 19, 100847. https://doi.org/10.1016/j.fochx.2023.100847

Published
2026-05-01
How to Cite
Lunnadiyah Aprilia, R., & Laila, A. N. (2026). Response of Four Lettuce Varieties to the NFT Hydroponic System with 3% H2O2 Application . JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), 8(2), 696 -. https://doi.org/10.36378/juatika.v8i2.5432
Abstract viewed = 13 times
PDF downloaded = 6 times