Biochemical Quality of Green Lettuce (Lactuca sativa L.) NFT Hydroponic in Various Types and Concentrations of Liquid Organic Fertilizer
Abstract
Green lettuce (Lactuca sativa L.) is a highly nutritious vegetable with increasing demand, making hydroponic cultivation using the nutrient film technique (NFT) a strategic option. However, reliance on AB Mix solution may lead to excessive nitrate accumulation, reducing food safety and the plant's biochemical quality. This study aimed to evaluate the effects of liquid organic fertilizer (POC) type and concentration on the chlorophyll, crude fiber, vitamin C, and nitrate content of lettuce grown hydroponically using NFT. The experiment used a split-plot design, with four POC types (Hantu, DI Grow, NASA, and Ribost) as the main-plot factor and four POC concentrations (0, 10, 20, and 30 mL/L) as the subplot factor. The experiment was replicated three times. POC concentration significantly affected chlorophyll content, with the highest value recorded at 30 mL/L (2.24 mg/L). Ribost produced the highest crude fiber content (0.97%), while DI Grow at 30 mL/L produced the highest vitamin C content (23.8 mg/100 g) and reduced nitrate levels from 3,511.0 to 2,782.1 mg/kg, below the European Union's safe threshold of 4,000 mg/kg. DI Grow at 30 mL/L is recommended as a partial substitute for AB Mix for hydroponic growers seeking to produce green lettuce with improved biochemical quality and lower nitrate levels.
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Afmerta, D., Ezward, C., & Mashadi. (2019). Uji pemberian pupuk Iskandar Muda (PIM) organik terhadap pertumbuhan dan produksi tanaman sawi (Brassica juncea L.). Jurnal Agronomi Tanaman Tropika (JUATIKA), 1(2), 1–11.
Ali, A. (2020). Nitrate assimilation pathway in higher plants: Critical role of nitrogen signalling and utilization. Plant Science Today, 7(2), 157–163. https://doi.org/10.14719/PST.2020.7.2.637
Badan Pusat Statistik. (2023). Produksi tanaman sayuran. https://www.bps.go.id/id/statistics-table/2/NjEjMg==/produksi-tanaman-sayuran.html
Barber, T. M., Kabisch, S., Pfeiffer, A. F. H., & Weickert, M. O. (2020). The health benefits of dietary fibre. Nutrients, 12(10), 3209. https://doi.org/10.3390/nu12103209 PubMed
Castro, J. C., Castro, C. G., & Cobos, M. (2023). Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway. Frontiers in Plant Science, 14, 1099829. https://doi.org/10.3389/fpls.2023.1099829
Choi, S., Colla, G., Cardarelli, M., & Kim, H. J. (2022). Effects of plant-derived protein hydrolysates on yield, quality, and nitrogen use efficiency of greenhouse grown lettuce and tomato. Agronomy, 12(5), 1018. https://doi.org/10.3390/agronomy12051018
Chou, E. Y., Schuetz, M., Hoffmann, N., Watanabe, Y., Sibout, R., & Samuels, A. L. (2018). Distribution, mobility, and anchoring of lignin-related oxidative enzymes in Arabidopsis secondary cell walls. Journal of Experimental Botany, 69(8), 1849–1859. https://doi.org/10.1093/jxb/ery067 PubMed Central (PMC)
Chowdhury, M., Samarakoon, U. C., & Altland, J. (2024). Evaluation of hydroponic systems for organic lettuce production in controlled environment. Frontiers in Plant Science, 15, 1401089. https://doi.org/10.3389/fpls.2024.1401089 DOI
Colla, G., Cardarelli, M., Bonini, P., & Rouphael, Y. (2017). Foliar applications of protein hydrolysate, plant and seaweed extracts increase yield but differentially modulate fruit quality of greenhouse tomato. HortScience, 52(9), 1214–1220.
European Commission. (2023). Commission Regulation (EU) 2023/915 of 25 April 2023 on maximum levels for certain contaminants in food and repealing Regulation (EC) No 1881/2006. Official Journal of the European Union, 119, 103–157.
Hönig, M., Plíhalová, L., Husičková, A., Nisler, J., & Doležal, K. (2018). Role of cytokinins in senescence, antioxidant defence and photosynthesis. International Journal of Molecular Sciences, 19(12), 4045. https://doi.org/10.3390/ijms19124045 PubMed
Hooks, T., Masabni, J., Ganjegunte, G., Sun, L., Chandra, A., & Niu, G. (2022). Salt tolerance of seven genotypes of zoysiagrass (Zoysia spp.). Technology in Horticulture, 2(1), 1–7.
Huang, B., Song, J., Ding, H., & Jiang, D. (2025). Dietary exposure assessment of nitrate in Shandong population in China. Food Additives & Contaminants: Part A, 42. https://doi.org/10.1080/19440049.2025.2581165
Irawati, T., & Slamet, D. A. N. (2017). Pengaruh umur bibit dan umur panen terhadap pertumbuhan dan produksi hidroponik NFT tanaman selada (Lactuca sativa L.) varietas Grand Rapids. Jurnal Agrinika, 1, 21–26.
Jokinen, K., Salovaara, A.-K., Wasonga, D. O., Edelmann, M., Simpura, I., & Mäkelä, P. S. A. (2022). Root-applied glycinebetaine decreases nitrate accumulation and improves quality in hydroponically grown lettuce. Food Chemistry, 366, 130558. https://doi.org/10.1016/j.foodchem.2021.130558
Khan, K. A., Yan, Z., & He, D. (2018). Impact of light intensity and nitrogen of nutrient solution on nitrate content in three lettuce cultivars prior to harvest. Journal of Agricultural Science, 10(6), 99–109. https://doi.org/10.5539/jas.v10n6p99
Li, T., Wu, Q., Zhen, Q., Xiao, X., Yang, Q., & Zhan, X. (2024). Effects of light intensity on growth and nitrate metabolism of lettuce in a plant factory. Journal of Plant Nutrition, 47(8), 1234–1247. https://doi.org/10.1080/01904167.2024.2311654
Li, Y., Cao, T., Guo, Y., Grimm, B., Li, X., Duanmu, D., & Lin, R. (2025). Regulatory and retrograde signaling networks in the chlorophyll biosynthetic pathway. Journal of Integrative Plant Biology, 67(4), 887–911. https://doi.org/10.1111/jipb.13837 Wiley Online Library
Liang, G., & Zhang, Z. (2020). Reducing the nitrate content in vegetables through joint regulation of short-distance distribution and long-distance transport. Frontiers in Plant Science, 11, 1079. https://doi.org/10.3389/fpls.2020.01079
Mampholo, B. M., Maboko, M. M., Soundy, P., & Sivakumar, D. (2018). Variety-specific responses of lettuce grown in a gravel-film technique closed hydroponic system to N supply on yield, morphology, phytochemicals, mineral content and safety. Journal of Integrative Agriculture, 17(11), 2447–2457. https://doi.org/10.1016/S2095-3119(18)62007-6
Mohammed, A. M. D., Basha, A. A. A., Youssef, M. A., & Bakr, A. A. A. (2025). Evaluation of different organic nitrogen nutrition for lettuce in hydroponic system. SVU-International Journal of Agricultural Sciences, 7(3), 1–9.
Muis, A., Hidayah, D. N., Elliza, N., & Shah, Z. (2022). Effect of hydroponic and conventional production systems on plant growth performance and nitrate content of green coral lettuce (Lactuca sativa var. Crispa). [Nama jurnal tidak tercantum pada data sumber], 1, 51–56.
Naim, M. A. (2022). Studi metode korelasi pengetahuan masyarakat tentang manfaat vitamin C dan konsumsi suplemen vitamin C di Desa Jipo Kecamatan Kepohbaru Kabupaten Bojonegoro. Repository Universitas Nahdlatul Ulama Sunan Giri. https://repository.unugiri.ac.id/id/eprint/1933/
Ohse, S., Dourado-Neto, D., Manfron, P. A., & Santos, O. S. (2001). Quality of lettuce cultivars grown in hydroponic solution. Scientia Agricola, 58(1), 181–185. https://doi.org/10.1590/S0103-90162001000100027
Pedrinho, D. R., Bimbato, S. M., Bono, J. A. M., Matias, R., Aguiar, E. B., Araújo, G. M., Sauer, A. V., & Oliveira, A. S. (2021). Nitrate content in lettuce grown in different production systems. Research, Society and Development, 10(3), e9210313049. https://doi.org/10.33448/rsd-v10i3.13049
Rasouli, F., Amini, T., Asadi, M., Hassanpouraghdam, M. B., Aazami, M. A., Ercisli, S., Skrovankova, S., & Mlcek, J. (2022). Growth and antioxidant responses of lettuce (Lactuca sativa L.) to arbuscular mycorrhiza inoculation and seaweed extract foliar application. Agronomy, 12(2), 401. https://doi.org/10.3390/agronomy12020401
Rouphael, Y., Carillo, P., Garcia-Perez, P., Cardarelli, M., Senizza, B., Miras-Moreno, B., Colla, G., & Lucini, L. (2022). Plant biostimulants from seaweeds or vegetal proteins enhance the salinity tolerance in greenhouse lettuce by modulating plant metabolism in a distinctive manner. Scientia Horticulturae, 305, 111368. https://doi.org/10.1016/j.scienta.2022.111368
Ruangrak, E., Salaemae, N., Sornnok, S., & Htwe, N. M. P. S. (2023). Effect of pre-harvest periods by replacing nutrient solution with tap water on nitrate and quality in hydroponic lettuce. ASEAN Journal of Scientific and Technological Reports, 26(1), 76–83. https://doi.org/10.55164/ajstr.v26i1.247666
Saragih, R. S., Rosmayati, & Hanum, H. (2026). The effect of LOF concentration in AB MIX on the growth of oil palm seedlings (Elaeis guineensis Jacq.) wick system. Jurnal Agronomi Tanaman Tropika (JUATIKA), 8(2), 495–503. https://doi.org/10.36378/juatika.v8i2.5137
Setiawan, F. A., Dekrityana, L. C. E. N. S., Salundik, & Komala, I. (2024). Utilization of liquid organic fertilizer from cow and chicken manure, Indigofera on hydroponic lettuce, and consumer perception. Mimbar Agribisnis, 10(2), 2924–2935. https://doi.org/10.25157/ma.v10i2.14578
Shigeto, J., & Tsutsumi, Y. (2016). Diverse functions and reactions of class III peroxidases. Applied Microbiology and Biotechnology, 100, 1395–1402.
Wang, L., Ning, S., Zheng, W., Guo, J., Li, Y., Li, Y., Chen, X., Ben-Gal, A., & Wei, X. (2023). Performance analysis of two typical greenhouse lettuce production systems: Commercial hydroponic production and traditional soil cultivation. Frontiers in Plant Science, 14, 1165856. https://doi.org/10.3389/fpls.2023.1165856
Yang, X., Feng, L., Zhao, L., Liu, X., & Huang, D. (2017). Effect of glycine nitrogen on lettuce growth under soilless culture: A metabolomics approach to identify the main changes occurred in plant primary and secondary metabolism. Journal of the Science of Food and Agriculture. https://doi.org/10.1002/jsfa.8482
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