Kinetin Concentration Test On Growth Castury Orange (Citrus microcarpa B.) On Medium Woody Plant Medium (WPM)

M. Supriadi Universitas Islam Kuantan Singingi
Tri Nopsagiarti Universitas Islam Kuantan Singingi
Chairil Ezward Universitas Islam Kuantan Singingi

DOI: https://doi.org/10.36378/internationalconferenceuniks.v0i0.2816

Keywords: Explants, Kasturi Orange, Kinetin, Concentration

Abstract

The development of kasturi oranges is still little done because it is easily attacked by citrus plant pests, especially in humid conditions. Therefore, another solution can be to multiply the seeds with the tissue culture technique. The success of implementing tissue culture techniques is determined, among others, by the use of an appropriate media composition. Kinetin is one type of cytokinin growth regulator which is widely used for shoot propagation because it can stimulate the formation of shoots, with high concentrations not easily damaged when the media is sterilized. The purpose of this study was to examine the effect of kinetin administration on the growth of explants of Kasturi orange (Citrus microcarpa B). The design used in this study was a non-factorial Completely Randomized Design (CRD) consisting of 7 levels of treatment with 3 replications, namely: K0 (Without Kinetin administration), K1 (Kinetin administration 1.5 mg/I), K2 (Kinetin administration) 2 mg/I), K3 (Giving Kinetin 2.5 mg/I), K4 (Giving Kinetin 3 mg/I), K5 (Giving Kinetin 3.5 mg/I), and K6 (Giving Kinetin 4 mg/I). Thus this study consisted of 21 experimental units. Each unit consisted of 1 culture bottle, each containing 4 explants, 3 of which were sampled. The results showed that the administration of kinetin to explants of musk kasturi orange had a significant effect on root length parameters with the best treatment at K0 (without kinetin administration) with an average of 8.22 cm. However, there was no significant effect on the parameters of the age of shoot emergence, number of shoots, and number of leaves.

References

[1] Aini, Nur Syariffah. 2012. “Multiplikasi Tunas Jeruk Keprok Tawangmangu (Citrus nobilis) Dengan Variasi Konsentrasi IBA Dan Kinetin.” Journal of Chemical Information and Modeling 53(9):1689–99.

[2] Dewi, S., P., and S. Dyah. 2010. “Pengaruh Kinetin Terhadap Inisiasi Dan Pertumbuhan Tunas Pada Perbanyakan Tanaman Jarak Pagar (Jatropha curcas L) Secara In Vitro.” Agrin 14(1):29–36.

[3] Dwi, Sulichantini, and Ellok. 2016. “Pengaruh Konsentrasi Zat Pengatur Tumbuh Terhadap Regenerasibawang Putih (Allium sativum L) Secara Kultur Jaringan.” Agrifor XV(Vol 15, No 1 (2016): Maret):29–36.

[4] Gusti, Janniatul Nisaq. 2017. Induksi Kalus Beberapa Jenis Jeruk (Citrus Sp) Dengan Pemberian Beberapa Konsentrasi Picloram Secara In Vitro. Skripsi. Fakultas Pertanian. Universitas Andalas.

[5] Hardiyati, Triani, Iman Budisantoso, and Universitas Jenderal Soedirman. 2021. “Multiplikasi Tunas Pisang Ambon Dua Tandan Pada Pemberian Kinetin Dalam Kultur In Vitro.” 38(1):11–17. doi: 10.20884/1.mib.2021.38.1.890.

[6] Karjadi, A. K., A. Buchory. 2008. Pengaruh Auksin dan Sitokinin terhadap Pertumbuhan dan Perkembangan Jaringan Meristem Kentang Kultivar Granola. J. Hort, 18(4):380-384.

[7] Mahadi, Imam, Wan Syafi’i, and Suci Agustiani. 2015. “Kultur Jaringan Jeruk Kasturi (Citrus microcarpa) Dengan Menggunakan Hormon Kinetin Dan Naftalen Acetyl Acid (Naa).” Jurnal Dinamika Pertanian XXX(1):37–44.

[8] Maisarah, Putri, and Mayta Isda Novaliza. 2021. “Induksi Tunas Dari Eksplan Epikotil Jeruk Kasturi (Citrus microcarpa B) Dengan Penambahan BAP Dan Kinetin Secara In Vitro.” Jurnal Ilmiah Ilmu-Ilmu Hayati, 6(2527–3221):138–46.

[9] Nisa, and Rodinah. 2005. Kultur Jaringan Beberapa Kultivar Buah Pisang (Musa paradisiaca L.) Dengan Pemberian Campuran NAA Dan Kinetin. Jurnal Bioscientiae, 2(2) , 23-36.

[10] Ramli, F., Durani, Siswadi, Barianto, N. Febridar, F. Irawan, Purwolelono, A. Suprianto, and Setiono. 2012. Jeruk Varietas Kalamansi FR. Laporan. Dinas Pertanian, Perkebunan dan Kehutanan Kabupaten Bengkulu.

[11] Sundari, Lidya, A. M. luthfi Siregar, and Hanafiah Sofiah Diana. 2015. “Respon Eksplan Nodus Dalam Inisiasi Tunas Mikro Tanaman Karet (Hevea brasiliensis muell Arg.) Dalam Medium MS.” Jurnal Agroekoteknologi Universitas Sumatera Utara 3(1):103043. doi: 10.32734/jaet.v3i1.9387.

[12] Wahyuni, Dewi, and A. Fitrianingsih. 2009. “Tekhnik Pemberian Benzyl Amino Purin Untuk Memacu Pertumbuhan Kalus Dan Tunas Pada Kotiledon Melon (Cucumis melo L,).” Tekhnik Pertanian 14(2):50–53.

[13] Wahyuni, Yana Sri. 2020. Respon Jeruk Kasturi ( Citrus microcarpa Bunge ) Dengan Pemberian Kinetin Dan Naa Secara In-Vitro Respon Jeruk Kasturi ( Citrus microcarpa Bunge ) Dengan Pemberian Kinetin Dan Naa Secara In-Vitro. Skripsi. Fakultas Pertanian. Uin Suska Riau. Pekanbaru.

[14] Widyastuti, Kiki. 2017. Pengaruh Kombinasi NAA (Naphtalen Acetic Acid) Dan BAP (Benzil Amino Purine) Terhadap Induksi Tunas Aksilar Tanaman Balsam (Polygala paniculata L.) Secara In Vitro. Skripsi. Jurusan Biologi Fakultas Sains dan Teknologi. Universitas Islam Negeri Maulana Malik Ibrahim. Malang.

[15] Zulkarnain. 2009. Kultur Jaringan Tanaman, Solusi Perbanyakan Tanaman Budi Daya. PT. Bumi Aksara. Jakarta.

Published

2022-12-29

Issue

2022: Proceeding of International Conference on Science and Technology

Section

Articles

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